151173

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151173

Sigma-Aldrich

 

Ninhydrin

ACS reagent

Synonym:1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate
CAS Number:485-47-2
Linear Formula:C9H6O4
Molecular Weight:178.14
Beilstein Registry Number:1910963
EC Number:207-618-1
MDL number:MFCD00003791
PubChem Substance ID:24849098

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References

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Description

ApplicationUsed for the detection of free amino groups in amino acids, peptides and proteins
 Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines.1,2 Reaction with sarcosine or proline gives azomethine ylides.3
Packaging25, 100 g in glass btl

Properties

gradeACS reagent
identification and melting pointpasses test
color white to brownish-white, crystals
mp250 °C (dec.)(lit.)
solubilityH2O:  passes test
suitabilitypasses test for sensitivity to amino acids

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXn
Risk Statements22-36/37/38
Safety Statements26
WGK Germany3
RTECSNK5425000

References

Cited Reference1. Anal. Biochem. 211, 240, 242, (1993)
 2. Tet. 34, 1285, (1978)
 3. Gazz. Chim. Ital. 123, 137, (1993)
referenceMoore, S., Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction. J. Biol. Chem. 243, 6281-6283, (1968) Abstract
 Kaiser, E., Kaiser test. Anal. Biochem. 34, 595, (1970)
 Samejima, K., Reagent for detecting and assaying peptides, amino acids, amines and amino sugars. Anal. Biochem. 42, 222, 237, (1971)
 Schoenberg, A., Singer, E., Review. Tet. 34, 1285, (1978)
 James, L.B., Amino acid analysis: ninhydrin reaction with titanous chloride. J. Chromatogr. Sci. 152, 298, (1978)
MerckMerck 13,6582